GATA-2/3 rabbit pAb

SKU: orb768355

Description

Rabbit polyclonal antibody to GATA-2/3

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Dilution range: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.
• Reactivity: Human, Mouse

Key Properties

• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human GATA3. AA range:274-323
• Molecular Weight: 48kD
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Anti-GATA3 antibody, anti-Trans-acting T-cell-specific transcription factor GATA-3 antibody, anti-GATA-binding factor 3 antibody, anti-GATA2 antibody, anti-Endothelial transcription factor GATA-2 antibody, anti-GATA-binding protein 2 antibody

Immunofluorescence analysis of Hela cell. 1, GATA-2/3 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). HSP70 Monoclonal Antibody (3G10) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of mouse-lung lysis using GATA-2/3 antibody. Antibody was diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit.

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:100 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).