Goat anti-GATA3 Antibody

• Catalog Number: orb18417
• Category: Antibodies
• Description: Goat polyclonal antibody to GATA3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Bovine, Canine, Human, Mouse, Porcine, Rat
• Dilution range: ELISA: 1:64000, WB: 0.3-1 μg/ml
• Conjugation: Unconjugated
• MW: 48.0; 47.9
• Target: GATA3
• Entrez: 2625
• Protein Sequence: EVTADQPRWVSHH
• RRID: AB_10750566
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti GATA3 antibody, anti GATA binding protein 3 a
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in lysates of cell line MOLT4 (calculated MW of 48.0 according to NP_001002295.1 and 47.9kDa according to NP_002042.1). Recommended for use at 1-3 μg/ml.Experiment Notes: Immunohistochemistry: In paraffin embedded Human Tonsil, stains nuclei of some cells in the interfollicular area and in germinal centres. Recommended concentration, 3ug/ml. Data obtained with a previous batch.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of staining of Human Tonsil (Lane1) and Mouse Kidney (Lane2) lysate using GATA3 antibody

Western blot analysis of staining of Human Tonsil (Lane1) and Mouse Kidney (Lane2) lysate using GATA3 antibody

Immunohistochemical staining of Human tonsil using GATA3 antibody