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Catalog Number | orb627236 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to GAPDH |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | ELISA, FC, IF, IHC, IP, WB |
Reactivity | Human, Mouse, Rat, Zebrafish |
Isotype | IgG1 |
Immunogen | glyceraldehyde-3-phosphate dehydrogenase |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:5000-1:20000; IP: 1:3000-1:20000; IHC: 1:50-1:500; IF: 1:20-1:200 |
Form/Appearance | liquid |
Purity | ≥95% as determined by SDS-PAGE |
Conjugation | Unconjugated |
MW | 36 kDa |
Target | GAPDH |
UniProt ID | P04406 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | G3PD, GAPD, GAPDH Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
IP Result of anti-GAPDH (IP: orb627236, 4 ug; Detection: orb627236 1:3000) with Hela cells lysate 3000 ug.
HeLa cells were subjected to SDS PAGE followed by western blot with orb627236 (GAPDH antibody) at dilution of 1:10000.
Immunohistochemistry of paraffin-embedded human kidney tissue slide using orb627236 (GAPDH Antibody) at dilution of 1:100.
WB | |
Canine, Gallus, Monkey, Porcine, Rabbit, Sheep | |
Hamster, Human, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC-Fr, IHC-P, WB | |
Mouse, Rat | |
Hamster, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC-Fr, IHC-P, WB | |
Gallus, Monkey, Zebrafish | |
Human, Mouse, Rat | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC-P, IP, WB | |
Bacteria, Bovine, Canine, Drosophila, E. coli, Fish, Gallus, Hamster, Human, Insect, Mammal, Monkey, Mouse, Other, Plant, Porcine, Rabbit, Rat, Yeast, Zebrafish | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC, WB | |
Canine, Human, Mouse, Porcine | |
Goat | |
Polyclonal | |
Unconjugated |