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Description
Research Area
Metabolism Research
Images & Validation
−| Tested Applications | IF, IHC-P, WB |
|---|---|
| Reactivity | Canine, Gallus, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep, Yeast |
| Application Notes |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG1 |
| Clone No. | B4D7 |
| Immunogen | Synthetic Peptide |
| Molecular Weight | 37 kDa |
| Purification | The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen |
| Conjugation | Unconjugated |
Customer Validated Data
− The data below is submitted by researchers worldwide. We provide this transparency to help you decide if this product works for your specific application or species, even if we haven't tested it ourselves.
Success by Application
Reactivity Distribution
Latest Experiments
6 ResultsDilution
Sample
Summary
WB
Human
1:2000
Human umbilical vein endothelial cells (HUVECs)
Cells are lysed in lysis buffer...
WB
Mouse
-
Liver and gastrocnemius muscle tissues
Tissues are lysed in buffer with...
WB
Rat
-
Rat heart tissue
Heart tissues were homogenized in RIPA...
WB
Rat
1:5000
Rat brain tissues
Tissues are lysed in RIPA buffer...
WB
Rat
1:5000
Brain tissues (prefrontal cortex, hippocampus, and striatum)
Tissues were lysed in RIPA buffer...
Previous
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid solution |
| Buffer/Preservatives | Liquid in PBS, pH 7.4, containing 0.02% Sodium Azide as preservative and 50% Glycerol. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Hazard Information | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
| Disclaimer | For research use only |
Alternative Names
−GAPDH; GAPD; CDABP0047; OK/SW-cl.12; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH
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Quality Guarantee
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Quick Database Links
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IF
Immunofluorescence











































