GAPDH Antibody

SKU: orb166925

Description

Mouse monoclonal antibody to GAPDH.

Research Area

Metabolism Research

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: WB: 1:2000-10000, IHC-P: 1:25, IF/ICC: 1:25
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: B3C32C32
• Immunogen: GAPDH recombinant protein is used to produce this monoclonal antibody.
• Target: GAPDH
• Molecular Weight: 36053
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti GAPDH antibody, anti GAPD antibody, anti Glyceraldehyde-3-phosphate dehydrogenase antibody, anti Peptidyl-cysteine S-nitrosylase GAPDH antibody

All lanes: Anti-GAPDH Antibody at 1:1000 dilution + C6 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/15000 dilution.Observed band size: 36KDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody at 1:1000 dilution. Lane 1: A431 whole cell lysates. Lane 2: C6 whole cell lysates. Lane 3: Hela whole cell lysates. Lane 4: HUVEC whole cell lysates.Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-GAPDH Antibody at 1:8000 dilution. Lane 1: Hela whole cell lysates. Lane 2: A549 whole cell lysates. Lane 3: COS-7 whole cell lysates. Lane 4: mouse brain lysates. Lane 5: C6 whole cell lysates. Lane 6: NIH/3T3 whole cell lysates. Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Fluorescent image of Hela cells stained with XAF1 GAPDH Antibody. diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded H.kidney section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Immunohistochemical analysis of paraffin-embedded H.stomach section using GAPDH Antibody. diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.

Western blot analysis of anti-GAPDH Monoclonal Antibody in CEM cell line lysates (35 μg/lane). GAPDH (arrow) was detected using the purified Mab.

Quick Database Links

Gene Symbol

GAPDH

UniProt

P04406

RefSeq (Protein):NP_001243728.1, NP_002037.2

Jie Wu 1, Hongya Wu 2, Bin Zhou 2, Xuefei He 1, Zhengjia Kang 1, Tiantian Chen 1, Gaoqin Liu 1, Peirong Lu 1 Effect of Topical Application of Dexamethasone on CC Chemokine Receptor 3 Expression in Human Pterygium J Ocul Pharmacol Ther, (2025)

PubMed: 40833917

Applications

WB

Reactivity

Human