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Description
Research Area
Images & Validation
−| Tested Applications | FC, WB |
|---|---|
| Reactivity | Human |
| Predicted Reactivity | Bovine, Hamster, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit Ig |
| Immunogen | This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A. |
| Target | GADD45A |
| Molecular Weight | 18 kDa |
| Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of GADD45A Antibody in Y79 cell line lysates (35 ug/lane)

Flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of GADD45A using rabbit polyclonal GADD45A Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.
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Protocol Information
GADD45A Antibody (orb1264605)
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