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Catalog Number | orb1931458 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB16521 |
Tested applications | FC, WB |
Predicted Reactivity | Hamster, Mouse, Rat |
Reactivity | Human |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 18336 Da |
Target | This GADD45A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 133-165 amino acids from the C-terminal region of human GADD45A. |
UniProt ID | P24522 |
NCBI | NP_001915.1, NP_001186670.1, NP_001186671.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Growth arrest and DNA damage-inducible protein GAD Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
GADD45A Antibody (C-term) flow cytometric analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of GADD45A Antibody (C-term) in Y79 cell line lysates (35 ug/lane). GADD45A (arrow) was detected using the purified Pab.
Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.