GADD45A Antibody (N-term)

SKU: orb1431404

Description

Rabbit polyclonal antibody to GADD45A.

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:1000, IHC-P: 1:100, FC: 1:10~50
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Clone No.: RB16520
• Immunogen: Synthetic Peptide
• Target: GADD45A
• Molecular Weight: 18336
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Disclaimer: For research use only

Alternative Names

Growth arrest and DNA damage-inducible protein GADD45 alpha, DNA damage-inducible transcript 1 protein, DDIT-1, GADD45A, DDIT1, GADD45

Formalin-fixed and paraffin-embedded H.tonsil tissue reacted with GADD45A Antibody (N-term).

GADD45A Antibody (N-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of anti-GADD45A Antibody (N-term) in Y79 cell line lysates (35 ug/lane). GADD45A (arrow) was detected using the purified Pab.

Western blot analysis of GADD45A (arrow) using rabbit polyclonal GADD45A Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GADD45A gene.

Quick Database Links

Gene Symbol

GADD45A

UniProt

P24522

RefSeq (Protein):NP_001186671.1, NP_001186670.1, NP_001915.1