FOXA2 Antibody

SKU: orb1247503

Description

FOXA2 Antibody

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60kDa band observed in Human Liver and Rat Lung lysates (calculated MW of 48.3kDa according to Human NP_710141.1 and 48.5kDa according to Rat NP_036875.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Prostate shows nuclear staining in the secretory cells of the gland. Recommended concentration: 4-8µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-GVYSRPIMNSS
• Target: FOXA2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FOXA2, HNF3B, forkhead box A2, TCF3B, MGC19807, hepatic nuclear factor-3-beta, hepatocyte nuclear factor 3, beta

orb1247503 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1247503 (3.8 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247503 (1 ug/ml) staining of Rat Lung lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FOXA2

UniProt

Q9Y261

RefSeq (Protein):NP_710141.1, NP_068556.2