FOXA2/HNF3B Antibody

SKU: orb18541

Description

Goat polyclonal antibody to FOXA2

Research Area

Developmental Biology, Epigenetics, Stem Cells

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 4-8 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 60kDa band observed in Human Liver lysates (calculated MW of 48.3kDa according to NP_068556.1and NP_710141.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17;277(20):17564-70.; PMID: 11875061). Recommended concentration: 1-3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: FOXA2 / HNF3B
• Protein Sequence: GVYSRPIMNSS
• Molecular Weight: 48.9; 48.3
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FOXA2 antibody, anti HNF3B antibody, anti forkhead box A2 antibody, anti TCF3B antibody, anti MGC19807 antibody, anti hepatic nuclear factor-3-beta antibody, anti hepatocyte nuclear factor 3, beta antibody

orb18541 staining (1ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18541 (1µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

orb18541 (3.8µg/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

FOXA2 / HNF3B

NCBI Gene ID

3170