Fibronectin Antibody

SKU: orb699544

Description

Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, IHC-P
• Dilution Range: ELISA: order Ab without BSA for coating,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Fibronectin antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG, kappa
• Clone No.: FN1/3029
• Immunogen: A portion of amino acids 467-595 from the human protein was used as the immunogen for the Fibronectin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

UniProt

P02751