Fibronectin 1 Rabbit Polyclonal Antibody

SKU: orb1294256

Description

Rabbit polyclonal antibody to Fibronectin (C-term)

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:500, Immunofluorescence 1:200–1:500, Immunohistochemistry (Paraffin) 1:100–1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: Fibronectin 1 / FN1 (C-term)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

All lanes: Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294256 overnight at 4°C.

Anti-Fibronectin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was incubated with orb1294256 overnight at 4°C.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: Fibronectin antibody (orb1294256).

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294256 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.

Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.

Quick Database Links

Gene Symbol

Fibronectin 1 / FN1 (C-term)