You have no items in your shopping cart.
Cart summary
Item 1 of 8
Item 1 of 8
Fibronectin 1 / FN1 (C-term)
Catalog Number: orb1294256
| Catalog Number | orb1294256 |
|---|---|
| Category | Antibodies |
| Description | Rabbit polyclonal antibody to Fibronectin (C-term) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IF, IHC, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit-IgG |
| Immunogen | Synthetic peptide / encompassing a sequence within the C-terminus region. |
| Dilution range | Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200 |
| Conjugation | Unconjugated |
| Target | Fibronectin 1 / FN1 (C-term) |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294256 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human hepatocellular carcinoma (HCC) labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded human breast carcinoma labeling Fibronectin with orb1294256 at 1/100.
Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with orb1294256 at 1/100.
Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294256 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
Anti-Fibronectin antibody at 1/1000 dilution. Lysates at 50 μg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was incubated with orb1294256 overnight at 4°C.
