FKBP5 Antibody

SKU: orb1257807

Description

FKBP5 Antibody

Research Area

Immunology & Inflammation, Signal Transduction

Images & Validation

• Tested Applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Application Notes:
  WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).
• Target: FKBP5
• Molecular Weight: Observed: 51kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AIG61, FKBP54, P54, PPIase, Ptg-1FKBP5, peptidyl-prolyl cis-trans isomerase FKBP5, 51 kDa FK506-binding protein, 51 kDa FKBP, 54 kDa progesterone receptor-associated immunophilin, FF1 antigen, FK506 binding protein 5, FKBP-51, HSP90-binding immunophilin, PPIase FKBP5, T-cell FK506-binding protein, androgen-regulated protein 6, peptidylprolyl cis-trans isomerase, rotamase

Western blot analysis of extracts of various cell lines, using FKBP5 antibody (orb1257807) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human colon using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

FKBP5

UniProt

Q13451