FKBP5 Antibody

• Catalog Number: orb1257807
• Category: Antibodies
• Description: FKBP5 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).
• Concentration: batch dependent
• Dilution range: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 51kDa
• Target: FKBP5
• UniProt ID: Q13451
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: AIG61, FKBP54, P54, PPIase, Ptg-1FKBP5, peptidyl-p
• Note: For research use only
• Application notes: WB: 1:200 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using FKBP5 antibody (orb1257807) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human colon using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human appendix using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human uterus using FKBP5 antibody (orb1257807) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HeLa cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC12 cells using FKBP5 antibody (orb1257807) at dilution of 1:100. Blue: DAPI for nuclear staining.