FKBP51 Recombinant Rabbit Monoclonal Antibody

SKU: orb612232

Description

FKBP51 Recombinant Rabbit Monoclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-1000, IHC-P=1:50-200, IHC-F=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: 6G6
• Immunogen: KLH conjugated synthetic peptide derived from human FKBP51
• Target: FKBP5
• Molecular Weight: 51 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FKBP 5; FKBP 51; FKBP 54; FKBP-5; FKBP-51; FKBP5; FKBP5_HUMAN; FKBP54; HSP90 binding immunophilin; HSP90-binding immunophilin; P54 antibody Peptidyl prolyl cis trans isomerase; Peptidyl-prolyl cis-trans isomerase FKBP5; Peptidylprolyl cis trans isomerase; PPIase; PPIase FKBP5; Ptg 10; Ptg10; Rotamase; T cell FK506 binding protein; 51 kDa FK506 binding protein 5; 51 kDa FK506 binding protein; 51 kDa FK506-binding protein; 51 kDa FKBP; 54 kDa progesterone receptor associated immunophilin; 54 kDa progesterone receptor-associated immunophilin; AIG 6; AIG6; Androgen regulated protein 6; Androgen-regulated protein 6; FF1 antigen; FK506 binding protein 5; FK506-binding protein 5; MGC111006; OTTHUMP00000016268.

Blank control: Daudi. Primary Antibody (green line): Rabbit Anti-FKBP51 antibody (orb612232), dilution: 1:50 cells, Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1:1000. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FKBP51) Monoclonal Antibody, Unconjugated (orb612232) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FKBP51) Monoclonal Antibody, Unconjugated (orb612232) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FKBP51) Monoclonal Antibody, Unconjugated (orb612232) at 1:50 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Daudi cell lysate, Lane 2: Hela cell lysate, Primary: Anti-FKBP51 (orb612232) at 1:500 dilution, Secondary: Goat Anti-Rabbit IgG - HRP at 1:5000 dilution, Predicted band size: 51 kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

FKBP5

UniProt

Q13451