Fibronectin 1/FN1 (N-term) Antibody

• Catalog Number: orb1294386
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Fibronectin
• Target: Fibronectin 1 / FN1 (N-term)
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit-IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Immunogen: Synthetic peptide / encompassing a sequence within the N-terminus region.
• Tested applications: IF, IHC, WB
• Dilution range: Western Blot 1:500 Immunofluorescence 1:200–1:500 Immunohistochemistry (Paraffin) 1:100–1:200
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only

All lanes: Anti-Fibronectin antibody at 1/500 dilution. Lysates at 60 µg per lane. This blot was produced using a 5% SDS-PAGE. Nitrocellulose membrane was then blocked with 3% BSA for an hour before being incubated with orb1294386 overnight at 4°C.

Immunofluorescence: cells were fixed with 4% paraformaldehyde for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at room temperature. Cells were stained with orb1294386 anti-Fibronectin antibody (red) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue labeling Fibronectin with orb1294386 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling Fibronectin with orb1294386 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Kidney carcinoma tissue labeling Fibronectin with orb1294386 at 1/100.

Immunohistochemical analysis of paraffin embedded Human Liver carcinoma tissue labeling Fibronectin with orb1294386 at 1/100.