FGFR2 Antibody

• Catalog Number: orb1929043
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB17696
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 92025 Da
• Target: This FGFR2 antibody is generated from rabbits immunized with a his tag recombinant protein of human FGFR2.
• UniProt ID: P21802
• NCBI: NP_001138388.1, NP_001138389.1, NP_075259.4, NP_001138391.1, NP_001138386.1, NP_000132.3, NP_001138385.1, NP_001138387.1, NP_001138390.1, NP_075418.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fibroblast growth factor receptor 2, FGFR-2, K-sam
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of FGFR2 Antibody in NCI-H460 cell line lysates (35 ug/lane). SFGFR2 (arrow) was detected using the purified Pab.

Confocal immunofluorescent analysis of FGFR2 Antibody with U251 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Western blot analysis of FGFR2 (arrow) using rabbit polyclonal FGFR2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the FGFR2 gene (Lane 2).

FGFR2-Antibody flow cytometric analysis of U251 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human lung carcinoma with FGFR2 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.