Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) Rabbit Polyclonal Antibody

SKU: orb5241

Description

Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: ELISA, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from human FGFR1 around the phosphorylation site of Tyr463 SE(p-Y)EL
• Target: FGFR1
• Molecular Weight: 88 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

FGFR1+FGFR2(phospho Tyr463/Tyr466); FGFR1+FGFR2(phospho Tyr463+Tyr466); FGFR2(phospho Y463+Y466); bFGF R; BFGFR; C FGR; CD 331; CD331; CD331 antigen; CEK; FGFBR; FGFR 1; Fibroblast growth factor receptor 1; FLG; FLG protein; FLJ14326; FLT 2; FLT2; Fms like tyrosine kinase 2; Fms related tyrosine kinase 2; Fms related tyrosine kinase 2 Pfeiffer syndrome; H2; H3; H4; H5; HBGFR; Heparin binding growth factor receptor; Hydroxyaryl protein kinase; KAL 2; KAL2; MFR; N SAM; N sam tyrosine kinase; Protein tyrosine kinase; Tyrosylprotein kinase; Basic fibroblast growth factor receptor 1.

Antigen: 1.phosphopeptide (blue), 0.2 ug/100 ul, 2.non-phosphopeptide (red), 0.2 ug/100 ul, Primary: Antibody, 1:200, 1:400, 1:800, 1:1600, 1:3200, 1:6400, 1:12800, Secondary:HRP conjugated Goat Anti-Rabbit igG at1:5000, TMB staining: Read the data in Microplate Reader by 450nm.

Paraformaldehyde-fixed, paraffin embedded (Human glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human laryngeal carcinoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463 Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Phospho-FGFR1+FGFR2 (Tyr463/Tyr466)) Polyclonal Antibody, Unconjugated (orb5241) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: U87MG (Human) Cell Lysate at 30 ug, Primary: Anti-Phospho-FGFR1+FGFR2 (Tyr463/Tyr466) (orb5241) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 88 kD, Observed band size: 73 kD.

Quick Database Links

Gene Symbol

FGFR1

UniProt

P11362