Fascin Antibody

• Catalog Number: orb749792
• Category: Antibodies
• Description: Fascin/FSCN1 binds beta-catenin and colocalizes with it at the leading edges and borders of epithelial and endothelial cells. The role of FSCN1 in regulating cytoskeletal structures for the maintenance of cell adhesion, coordinating motility and invasion through interactions with signalling pathways is an active area of research especially from the cancer biology perspective. It localizes to actin-rich protrusions at the cell surface called filopodia. FSCN1 localizes to invadopodia, membrane protrusions formed at the adherent cell surface that facilitate extracellular matrix (ECM) invasion, this provide a potential molecular mechanism for how fascin increases the invasiveness of cancer cells since fascin expression is upregulated in a spectrum of cancers. [Wiki]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: FSCN1/418
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Mouse IgG2b, kappa
• Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.
• Dilution range: Western blot: 1-2ug/ml,Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 0.5-1ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This Fascin antibody is available for research use only.
• UniProt ID: Q16658
• Storage: Store the Fascin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.