Fascin Antibody

SKU: orb2639200

Description

Recognizes a protein of 55kDa, which is identified as fascin-1. Its actin binding ability is regulated by phosphorylation. Antibody to fascin-1 is a very sensitive marker for Reed-Sternberg cells and variants in nodular sclerosis, mixed cellularity, and lymphocyte depletion Hodgkin's disease. It is uniformly negative in lymphoid cells, plasma cells, and myeloid cells. Fascin-1 is also expressed in dendritic cells. This marker may be helpful to distinguish between Hodgkin lymphoma and non-Hodgkin lymphoma in difficult cases. Also, the lack of expression of fascin-1 in the neoplastic follicles in follicular lymphoma may be helpful in distinguishing these lymphomas from reactive follicular hyperplasia in which the number of follicular dendritic cells is normal or increased. Antibody to fascin-1 has been suggested as a prognostic marker in neuroendocrine neoplasms of the lung as well as in ovarian cancer. Fascin-1 expression may be induced by Epstein-Barr virus (EBV) infection of B cells with the possibility that viral induction of fascin in lymphoid or other cell types must also be considered in EBV-positive cases.

Images & Validation

• Tested Applications: FACS, IF, IHC-P, WB
• Dilution Range: Flow cytometry: 1-2ug/10^6 cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Fascin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2a, kappa
• Clone No.: FSCN1/417
• Immunogen: Full length recombinant human protein was used as the immunogen for the Fascin antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 1 mg/ml in 1X PBS; rAlbumin free, sodium azide free
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Western blot of HeLa cell lysate using Fascin antibody (clone FSN1/417). Predicted molecular weight: 55 kDa.

IHC: Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with Fascin antibody (clone FSCN1/417).

IHC: Formalin-fixed, paraffin-embedded human testicular carcinoma stained with Fascin antibody (clone FSCN1/417).

IHC: Formalin-fixed, paraffin-embedded human ovarian carcinoma stained with Fascin antibody (clone FSCN1/417).

Immunofluorescent staining of permeabilized human HeLa cells with Fascin antibody (clone FSCN1/417, green), Phalloidin (actin, red) and Hoescht (nucleus, blue).

Flow cytometry testing of PFA-fixed human K562 cells with Fascin antibody (clone FSCN1/417); Red = isotype control, Blue = Fascin antibody.

SDS-PAGE analysis of purified, BSA-free Fascin antibody (clone FSCN1/417) as confirmation of integrity and purity.

Quick Database Links

UniProt

Q16658