FAS Antibody

SKU: orb1249512

Description

FAS Antibody

Research Area

Cell Biology, Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in lysates of cell line MOLT4 (calculated MW of 37.7kDa according to NP_000034.1). Recommended concentration: 0.1-0.3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Tonsil and Small Intestine. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: KTCRKHRKENQGSH
• Target: FAS
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAS, Fas (TNF receptor superfamily, member 6), ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, APO-1 cell surface antigen, CD95 antigen, Fas AMA, Fas antigen, OTTHUMP00000020045, OTTHUMP00000020046, OTTHUMP00000020051, OTTHUMP00000059646, apoptosis antigen 1, tumor necrosis factor receptor superfamily member 6, tumor necrosis factor receptor superfamily, member 6

orb1249512 (0.1 ug/ml) staining of MOLT4 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1249512 ( ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1249512 (3.75 ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

FAS

UniProt

P25445

RefSeq (Protein):NP_690610.1, NP_690611.1, NP_000034.1