FASN Antibody (Center)

• Catalog Number: orb1939263
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 497CT15.2.5
• Tested applications: IF, WB
• Reactivity: Human
• Isotype: IgG1,κ
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1:2000, IF: 1:200, IF: 1:25, IF: 1:25, WB: 1:100, WB: 1:8000, WB: 1:1000
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 273427 Da
• Target: This FASN antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 942-973 amino acids from the Central region of human FASN.
• UniProt ID: P49327
• NCBI: NP_004095.4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Fatty acid synthase, [Acyl-carrier-protein] S-acet
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-FASN Antibody (Center) at 1:500-1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: Ramos whole cell lysate. Lane 5: HepG2 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 273 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-FASN Antibody (Center) at 1:8000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: 293 whole cell lysate. Lane 4: Ramos whole cell lysate. Lane 5: HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 273 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Anti- at 1:1000 dilution + HepG2 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 273 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

FASN Antibody (Center) western blot analysis in mouse brain tissue lysates (35 μg/lane). This demonstrates the FASN (Center) antibody detected the FASN (Center) protein (arrow).

Fluorescent confocal image of HepG2 cells stained with FASN (Center) Antibody. HepG2 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with FASN primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-mouse antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling FASN at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm HepG2 cell line. The nuclear counter stain is DAPI (blue).

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0. 1% Triton X-100 permeabilized U-2 OS ((human cervical epithelial adenocarcinoma cell line) cells labeling FASN at 1/25 dilution, followed by Dylight 488-conjugated goat anti-mouse IgG (35503) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm Hela cell line. The nuclear counter stain is DAPI (blue).