PDIA4 Antibody

SKU: orb1239828

Description

PDIA4 Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine
• Application Notes:
  ERp72 antibody can be used for detection of ERp72 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: ERp72 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human ERp72.The immunogen is located within the last 50 amino acids of ERp72.
• Target: PDIA4
• Molecular Weight: Predicted: 71 kDa Observed: 75 kDa
• Purification: ERp72 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: ERp72 antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Protein disulfide isomerase family A member 4, Endoplasmic reticulum resident protein 70, Endoplasmic reticulum resident protein 72, ERp70, ERp-72

Western blot analysis of ERp72 in human colon tissue lysate with ERp72 antibody at (A) 1 and (B) 2 µg/ml.

Immunohistochemistry of ERp72 in rat colon tissue with ERp72 antibody at 5 µg/mL.

Immunofluorescence of ERp72 in rat colon tissue with ERp72 antibody at 20 µg/mL.

Quick Database Links

Gene Symbol

PDIA4

UniProt

P13667

RefSeq (Protein):NP_004902