ERK1+2 Rabbit Polyclonal Antibody

SKU: orb1294321

Description

Rabbit polyclonal antibody to ERK1+ ERK2

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: Western Blot 1:1000-1:3000, Immunofluorescence 1:300-1:500
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / encompassing a sequence within the C-terminus region.
• Target: ERK1+2
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-ERK1+ERK2 antibody at 1/2000 dilution. Lysates at 30 µg per lane. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294321 overnight at 4°C.

ICC/IF: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294321 anti-ERK1+ERK2 antibody (red) at 1:200 and 4°C.

ICC/IF: cells were fixed with 4% PFA for 10 min at RT, permeabilized with 0.1% NP-40 for 10 min at RT then blocked with 5% BSA for 30 min at RT. Cells were stained with orb1294321 anti-ERK1+ERK2 antibody (red) at 1:200 and 4°C.

Sample: Neuron 2A, ERK1+2 antibody (orb1294321): 1-2000.

Quick Database Links

Gene Symbol

ERK1+2