Erk1/2 Antibody

SKU: orb1822436

Description

Rabbit Anti-Rat Erk1/2 Polyclonal

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: WB (1:1000), IHC (1:100), ICC/IF (1:100), FCM (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Drosophila, Frog, Gallus, Human, Mouse, Rat, Sheep
• Application Notes:
  A 1:1000 dilution was sufficient for detection of ERK1/2 in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A 35 residue synthetic peptide, corresponding to Rat Erk1 MAP kinase with the CGG spacer group added and the peptide coupled to KLH.
• Target: Erk1/2
• Molecular Weight: 42kDa (ERK2).
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide. Storage buffer changes when conjugated.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERK1, ERK2, ERT1, ERT2, MAP kinase 1, MAP kinase 2, MAPK1, MAPK2, MAPK3, p38, p40, p41, p41mapk, p42 MAPK, p44 ERK1, p44 MAPK, PRKM1, PRKM2, PRKM3

Quick Database Links

Gene Symbol

Erk1/2

UniProt

P21708

NCBI Gene ID

50689

RefSeq (Protein):NP_059043.1

Su-Ji Min, Hye-Won Hyun, Tae-Cheon Kang Leptomycin B attenuates neuronal death via PKA- and PP2B-mediated ERK1/2 activation in the rat hippocampus following status epilepticus Brain Research, 1670, 14 (2017)

PubMed: 28601633

Applications

WB

Reactivity

Rat