Phospho-ERK1/2 (Thr202 + Tyr204) Rabbit Polyclonal Antibody

SKU: orb5178

Description

Phospho-ERK1/2 (Thr202 + Tyr204) Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1μg /test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated Synthesised phosphopeptide derived from mouse p44/42 MAPK around the phosphorylation site of Thr202/Tyr204 FL(p-T)E(p-Y)VA
• Target: MAPK3
• Molecular Weight: 41 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ERK1 & ERK2 (p-T202/Y204); p-ERK1 & ERK2; phospho-ERK1 & ERK2; MK03_HUMAN; MAPK3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1 (ERK-1); Insulin-stimulated MAP2 kinase; MAP kinase isoform p44 (p44-MAPK); Microtubule-associated protein 2 kinase; p44-ERK1; 2.7.11.24; ERK1; PRKM3; MK01_HUMAN; MAPK1; MAP kinase 1; MAPK 1; ERT1; Extracellular signal-regulated kinase 2 (ERK-2); MAP kinase isoform p42 (p42-MAPK); Mitogen-activated protein kinase 2 (MAP kinase 2 | MAPK 2); ERK2; PRKM1; PRKM2; ERK; ERK-2; MAPK2; NS13; P42MAPK; p38; p40; p41; p41mapk; p42-MAPK; ERK-1; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; p44-MAPK;

Tissue/Cell: HUVEC cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-ERK1/2 (Thr202 + Tyr204)) polyclonal Antibody, Unconjugated (orb5178) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Blank control (blue line): U251 (fixed with 2% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature). Primary Antibody (green line): Rabbit Anti-phospho-ERK12 (Thr202 + Tyr204) antibody (orb5178), dilution: 3 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE, dilution: 1 µg/Test.

Paraformaldehyde-fixed, paraffin embedded (human Glioblastoma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 2 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1+2 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1 2 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK1+2 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat colon), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-ERK12 (Thr202 + Tyr204)) Polyclonal Antibody, Unconjugated (orb5178) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (phospho-ERK1/2 (Thr202 + Tyr204)) polyclonal Antibody, Unconjugated (orb5178) 1:100, 90 minutes at 37°C, followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-phospho-ERK1/2 (Thr202+Tyr204) Polyclonal Antibody, Unconjugated (orb5178) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

MAPK3

Zhang, Fengwei et al. Seasonal Expression of Oxytocin and Oxytocin Receptor in the Scented Gland of Male Muskrat (Ondatra zibethicus) Sci Rep, 7, 16627 (2017)

PubMed: 29192229