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Description
Research Area
Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1:500-1:3000; IHC: 1:50-1:500; IF: 1:10-1:100 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | mitogen-activated protein kinase 1 |
| Target | ERK1/2 |
| Molecular Weight | 38-44 kDa |
| Purity | ≥95% as determined by SDS-PAGE |
| Purification | Immunogen affinity purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunofluorescent analysis of Hela cells, using orb626802 (ERK1/2 Antibody) at 1:25 dilution. Blue pseudocolor = DAPI (fluorescent DNA dye).
HeLa cells were subjected to SDS PAGE followed by western blot with orb626802 (ERK1/2 antibody) at dilution of 1:1000.
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using orb626802 (ERK1/2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer (pH9).
Quick Database Links
Gene Symbol
ERK1/2
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
ERK1/2 Rabbit Polyclonal Antibody (orb626802)
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