ERK1/2 Mouse Monoclonal Antibody

SKU: orb500888

Description

ERK1/2 Mouse Monoclonal Antibody

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-5000, IHC-P=1:500-2000, IHC-F=1:500-2000, IF=1:500-2000, Flow-Cyt=1μg/Test
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG
• Clone No.: 3G4
• Immunogen: KLH conjugated synthetic peptide derived from human ERK1/2 (151-250/380aa)
• Target: MAPK3
• Molecular Weight: 43 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

ERK; ERK-1; ERT 2; ERT2; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; Insulin Stimulated MAP2 Kinase; Insulin-stimulated MAP2 kinase; MAP kinase 1; MAP kinase 3; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK 3; MAPK; MAPK1; Mapk3; MGC20180; Microtubule Associated Protein 2 Kinase; Microtubule-associated protein 2 kinase; Mitogen Activated Protein Kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 3; MK03_HUMAN; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; PRKM 3; PRKM3; Protein Kinase Mitogen Activated 3.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (ERK1) Monoclonal Antibody, Unconjugated (ascites of orb500888) at 1:2000 overnight at 4°C, followed by operating according to SP Kit (Mouse) instructions and DAB staining.

Sample: Lane 1: A431 (Human) Cell Lysate at 30 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Cerebrum (Rat) Lysate at 40 ug, Lane 4: SiHa (Human) Cell Lysate at 30 ug, Lane 5: MCF-7 (Human) Cell Lysate at 30 ug, Lane 6: HUVEC (Human) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 44/42 kD, Observed band size: 40/42 kD.

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-ERK1/2 (orb500888) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 43 kD, Observed band size: 38 kD.

Quick Database Links

Gene Symbol

MAPK3

UniProt

P27361