Erk1/2 Antibody

• Catalog Number: orb1787899
• Category: Antibodies
• Description: Mouse Monoclonal Antibody (Mab)
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: FC, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: 28-240 aa
• Dilution range: WB: 1:4000, FC: 1:25
• Conjugation: Unconjugated
• MW: 43136 Da
• Target: Purified His-tagged Erk1/2 protein was used to produced this monoclonal antibody.
• UniProt ID: P27361
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitogen-activated protein kinase 3, MAP kinase 3,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes: Anti-Erk1/2 Antibody at 1:4000 dilution. Lane 1: MCF-7 whole cell lysate. Lane 2: Jurkat whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 41 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Overlay histogram showing Jurkat cells stained (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight 488 Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1 μg/1x10^6 cells) used under the same conditions. Acquisition of >10000 events was performed.