ERK1 Rabbit mAb [KD Validated]

• Catalog Number: orb1951919
• Category: Antibodies
• Description: The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and Threonine sites mapping within a characteristic Thr- Glu-Tyr motif.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human ERK1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:250 IP 1:50 FC 1:50
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P27361
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: MAP kinase 3; MAPK 3; ERT2; ERK-1; p44-MAPK; p44-E
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using ERK1 Antibody.

Western blot analysis of ERK1 in NIH-3T3 cell lysate.