Raf1 Rabbit mAb [KD Validated]

• Catalog Number: orb1951842
• Category: Antibodies
• Description: Raf-1 is a MAP kinase kinase kinase (MAP3K) which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated Raf-1 can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2 which in turn phosphorylate to activate the serine/threonine specific protein kinases ERK1 and ERK2.
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Raf1
• Antibody Type: Recombinant Antibody
• Dilution range: WB 1:1000~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Conjugation: Unconjugated
• UniProt ID: P04049
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: C-RAF, C-Raf, CRAF, EC 2.7.11.1, Protein kinase ra
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Raf1 expression in K562 cell lysate.