RSK1(Phospho-Ser380) Rabbit mAb [KD Validated] Antibody

SKU: orb1727780

Description

Rsk1 is a member of a family of 90kDa ribosomal protein S6 kinases, which includes Rsk1, Rsk2 and Rsk3. These are broadly expressed serine / threonine protein kinases activated in response to mitogenic stimuli, including extracellular signal regulated protein kinases Erk1 and Erk2. Rsk1 is activated by MAPK in vitro and in vivo via phosphorylation. Active Rsks appear to play a major role in transcriptional regulation by translocating to the nucleus and phosphorylating c-Fos and CREB.

Images & Validation

• Tested Applications: ICC, IP, WB
• Dilution Range: WB:1:500~1:2000ICC/IF:1:50~1:200IP:1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Recombinant Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Immunogen: A synthesized peptide derived from human Phospho-RSK1(S380)
• Molecular Weight: 90kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

RSK; HU-1; RSK1; MAPKAPK1A; RPS6KA1;

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

Western blot analysis of Phospho-RSK1(S380) expression in A431 cell lysate treated with EGF.

Quick Database Links

UniProt

Q15418