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Catalog Number | orb750171 |
---|---|
Category | Antibodies |
Description | EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt] |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | EPM17-4 |
Tested applications | IF, IHC-P, WB |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Human recombinant partial protein (extracellular portion) was used as the immunogen for this EpCAM antibody. |
Dilution range | Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This EpCAM antibody is available for research use only. |
UniProt ID | P16422 |
Storage | Store the EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EpCAM antibody to be titered up or down for optimal performance. |
Expiration Date | 12 months from date of receipt. |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Canine, Feline, Human | |
Rabbit | |
Recombinant | |
Unconjugated |
IHC-P, WB | |
Human | |
Mouse | |
Recombinant | |
Unconjugated |
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