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Catalog Number | orb606388 |
---|---|
Category | Antibodies |
Description | EpCAM is frequently upregulated in carcinomas but is not expressed in cancers of non-epithelial origin. EpCAM expression is not limited to human colon carcinomas; in fact, EpCAM is expressed in a variety of human epithelial tissues, carcinomas, and progenitor and stem cells. [Wiki] |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | EGP40/2041R |
Tested applications | IHC-P, WB |
Reactivity | Canine, Feline, Human |
Isotype | Rabbit IgG, kappa |
Immunogen | A portion of amino acids 100-224 (extracellular domain) was used as the immunogen for the recombinant EpCAM antibody. |
Antibody Type | Primary Antibody |
Dilution range | Western blot:1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein A affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This recombinant EpCAM antibody is available for research use only. |
UniProt ID | P16422 |
Storage | Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | Titering of the recombinant EpCAM antibody may be required for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human thyroid carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
IHC testing of FFPE human basal cell carcinoma and recombinant EpCAM antibody (clone EGP40/2041R). Required HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
Western blot testing of human MCF7 cell lysate with recombinant EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
Western blot testing of human lung lysate with EpCAM antibody (clone EGP40/2041R). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).
SDS-PAGE analysis of purified, BSA-free recombinant EpCAM antibody (clone EGP40/2041R) as confirmation of integrity and purity.
IHC testing of FFPE dog bladder and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
IHC testing of FFPE cat small intestine and recombinant EpCAM antibody (clone EGP40/2041R). HIER: steam sections in pH9 EDTA for 10-20 min.
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P | |
Canine, Feline, Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Canine, Feline, Human | |
Rabbit | |
Recombinant | |
Unconjugated |