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Catalog Number | orb1294311 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to EpCAM antibody (Extracellular domain) |
Target | EpCAM / CD326 (Extracellular domain) |
Clonality | Polyclonal |
Species/Host | Rabbit |
Isotype | Rabbit-IgG |
Conjugation | Unconjugated |
Reactivity | Human |
Immunogen | Synthetic peptide / Extracellular domain corresponding to Human EpCAM |
Tested applications | IF, IHC, WB |
Dilution range | Western Blot 1:1000–1:2000 Immunofluorescence 1:200–1:400 Immunohistochemistry (Paraffin) 1:100-1:150 Flow Cytometry 1:50-1:200 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.
Product: EpCAM antibody; Catalog: orb1294311; Cell: MCF-7.
Cell: MCF-7 (DAPI: 1-300, Phalloidin: 1-300); EpCAM, (orb1294311): 1-200; Anti-rabbit 594: 1-500; NikonEpi-fluorescence (A-ken) 60x.
Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 μg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.
Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.