EpCAM Rabbit Polyclonal Antibody

SKU: orb1294311

Description

Rabbit polyclonal antibody to EpCAM antibody (Extracellular domain)

Research Area

Cancer Biology

Images & Validation

• Tested Applications: FC, IF, IHC, WB
• Dilution Range: Western Blot 1:1000–1:2000, Immunofluorescence 1:200–1:400, Immunohistochemistry (Paraffin) 1:100-1:150, Flow Cytometry 1:50-1:200
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Extracellular domain corresponding to Human EpCAM
• Target: EpCAM / CD326 (Extracellular domain)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.

Anti-EpCAM antibody - at 1/1000 dilution, Lysates at 40 µg per lane. This blot was produced using a 10% SDS-PAGE. Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294311 overnight at 4°C.

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: EpCAM antibody (extracellular domain) (orb1294311).

Cell: MCF-7 (DAPI: 1-300, Phalloidin: 1-300), EpCAM, (orb1294311): 1-200, Anti-rabbit 594: 1-500, NikonEpi-fluorescence (A-ken) 60x.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.

Immunohistochemical analysis of paraffin embedded human cancer tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

Immunohistochemical analysis of paraffin embedded mouse tissue labeling EpCAM with orb1294311 at 1/200 RT 1hr. Epitope Retrieval methods: Citrate Buffer, pH 6.0.

EpCAM antibody, orb1294311, Cell: MCF-7.

Quick Database Links

Gene Symbol

EpCAM / CD326 (Extracellular domain)