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EpCAM Antibody (Extracellular domain)

SKU: orb376650

Description

EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsIF, IHC-P, WB
Dilution RangeWestern blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
ReactivityHuman
Application Notes
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.

Key Properties

Antibody TypePrimary Antibody
HostMouse
ClonalityMonoclonal
IsotypeMouse IgG1, kappa
Clone No.EPM17-2
ImmunogenHuman partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.
PurificationProtein G affinity chromatography
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

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UniProt Details

No UniProt data available

Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC-P
Immunohistochemistry Paraffin
View Protocol
IF
Immunofluorescence
View Protocol

Available Sizes

Select a size below

20 μg
$ 370.00
100 μg
$ 750.00
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0

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