ENaC gamma Antibody

SKU: orb67569

Description

Rabbit Anti-Rat ENaC gamma Polyclonal

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), IHC-P (1:100)
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
• Target: ENaC gamma
• Molecular Weight: 83kDa
• Purification: Protein A Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

SCNN1G, Epithelial Sodium Channel gamma, Amiloride sensitive epithelial sodium channel gamma subunit, Amiloride sensitive sodium channel subunit gamma, Amiloride-sensitive sodium channel subunit gamma, BESC3, ENaC gamma subunit, ENaCG, ENaCgamma, Epithelial Na(+) channel subunit gamma, Epithelial Na+ channel subunit gamma, Gamma ENaC, Gamma NaCH, Gamma-ENaC, Gamma-NaCH, Nonvoltage gated sodium channel 1 subunit gamma, Nonvoltage-gated sodium channel 1 subunit gamma, PHA 1, PHA1, SCNEG, SCNN 1G, SCNNG_HUMAN, Sodium channel non voltage gated 1 gamma subunit, Sodium channel nonvoltage gated 1 gamma

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.

Quick Database Links

Gene Symbol

ENaC gamma

UniProt

P37091

NCBI Gene ID

24768

RefSeq (Protein):NP_058742