ENaC beta Antibody (Biotin)

SKU: orb152734

Description

Rabbit polyclonal to ENaC Beta (Biotin). The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport. Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption. ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta , and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate. The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure..

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, IP, WB
• Dilution Range: WB (1:1000), IHC (1:100)
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Application Notes:
  1 µg/ml was sufficient for detection of beta-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Produced against the C-terminal tail (amino acids 617-638) of rat beta ENaC (antibody designation 3755-2)
• Target: ENaC beta
• Molecular Weight: 87kDa
• Purification: Protein A Purified
• Conjugation: Biotin

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, 133.23 mM Chlorides, 9.55mM Phosphates, 9.55mM Sodium Bicarbonate.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Amiloride sensitive sodium channel subunit beta, Amiloride-sensitive sodium channel beta-subunit, Beta ENaC, Beta NaCH, ENaC beta, ENaCB, Epithelial Na(+) channel subunit beta, Epithelial Na+ channel beta subunit, Epithelial Na+ channel subunit beta, Epithelial sodium channel beta 2 subunit, Epithelial sodium channel beta 3 subunit, Nonvoltage gated sodium channel 1 beta subunit, Nonvoltage gated sodium channel 1 subunit beta, Nonvoltage-gated sodium channel 1 beta subunit, SCNEB, SCNN 1B, SCNN1B, Sodium channel nonvoltage gated 1 beta, Sodium channel nonvoltage gated 1 beta (Liddle syndrome)

Quick Database Links

Gene Symbol

ENaC beta

UniProt

P37090

NCBI Gene ID

24767

RefSeq (Protein):NP_036780