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ENaC gamma Rabbit Polyclonal Antibody
Description
Images & Validation
−| Tested Applications | ICC, IF, IHC-P, WB |
|---|---|
| Dilution Range | WB: 1:100-600, IHC-P: 1:50-300, IF/ICC: 1:50-300 |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | KLH conjugated synthetic peptide derived from human ENaC gamma. Please contact us for the exact immunogen sequence. The peptide is available as orb13781. |
| Target | ENaC gamma |
| Molecular Weight | 74 kDa |
| Purity | Polyclonal antibodies are purified by peptide affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | 10 mM PBS, 0.02% sodium azide |
| Concentration | - 100 μg (in 200 μl): 0.5 mg/ml- 200 μg (in 400 μl): 0.5 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
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Immunohistochemical staining of rat prostate tissue using anti-ENaC gamma (2.5 ug/ml)

IF image of guinea pig colon tissue using anti-ENaC gamma (2.5 ug/ml)

Western blot analysis of mouse kidney (lane 1), rat colon (lane 2), mouse trachea (lane 3), mouse lung (lane 4), rat liver (lane 5); mouse heart (lane 6) using anti-ENaC gamma (1 ug/ml)

IHC-P staining of mouse heart tissue using ENaC gamma antibody (2.5 ug/ml)

IF image of guinea pig colon tissue using anti-ENaC gamma (2.5 ug/ml)

Immunohistochemical staining of paraffin embedded guinea pig colon tissue using ENaC gamma antibody (2.5 ug/ml)
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Protocol Information
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Ye Gao 1, Fei Cao 2, Xinyi Tian 1, Qianping Zhang 3, Congcong Xu 1, Bowen Ji 1, Ye-An Zhang 1, Linan Du 1, Jun Han 1, Li Li 1, Siyu Zhou 1, Yuqiang Gong 1, Binyu Ying 1, Fang Gao-Smith 4, Shengwei Jin Inhibition the ubiquitination of ENaC and Na,K-ATPase with erythropoietin promotes alveolar fluid clearance in sepsis-induced acute respiratory distress syndrome Biomed Pharmacother, 174, 116447 (2024)
Applications
Reactivity
Han, Jun et al. Maresin Conjugates in Tissue Regeneration 1 improves alveolar fluid clearance by up-regulating alveolar ENaC, Na, K-ATPase in lipopolysaccharide-induced acute lung injury J. Cell. Mol. Med., 24, 4736-4747 (2020)
ENaC gamma Rabbit Polyclonal Antibody (orb389322)
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