ENaC beta Antibody: Biotin

SKU: orb383775

Description

Mouse monoclonal antibody against ENaC beta conjugated to Biotin.

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC, WB
• Dilution range: WB (1:1000); IHC (1:150)
• Reactivity: Mouse
• Application Notes:
  A 1:1000 dilution of SMC-240 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: 7B8
• Immunogen: Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
• Target: ENaC beta
• Molecular Weight: 87kDa
• Purification: Protein G Purified

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

SCNN1B antibody, Amiloride sensitive sodium channel subunit beta antibody, Amiloride-sensitive sodium channel beta-subunit antibody, Beta ENaC antibody, Beta NaCH antibody, ENaC beta antibody, ENaCB antibody, Epithelial Na(+) channel subunit beta antibody, Epithelial Na+ channel beta subunit antibody, Epithelial Na+ channel subunit beta antibody, Epithelial sodium channel beta 2 subunit antibody, Epithelial sodium channel beta 3 subunit antibody, Nonvoltage gated sodium channel 1 beta subunit antibody, Nonvoltage gated sodium channel 1 subunit beta antibody, Nonvoltage-gated sodium channel 1 beta subunit antibody, SCNEB antibody, SCNN 1B antibody, Sodium channel nonvoltage gated 1 beta (Liddle syndrome) antibody, Sodium channel nonvoltage gated 1 beta antibody

Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.

Quick Database Links

Gene Symbol

ENaC beta

UniProt

P37090

NCBI Gene ID

24767

RefSeq (Protein):NP_036780