ENaC beta Antibody: Biotin

• Catalog Number: orb383775
• Category: Antibodies
• Description: Mouse monoclonal antibody against ENaC beta conjugated to Biotin.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7B8
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: Synthetic peptide from the C-terminal of Rat ENaC beta (aa. 617-638)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000); IHC (1:150)
• Conjugation: Biotin
• MW: 87kDa
• Target: ENaC beta
• Entrez: 24767
• UniProt ID: P37090
• NCBI: NP_036780
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: SCNN1B antibody, Amiloride sensitive sodium channe
• Note: For research use only
• Application notes: A 1:1000 dilution of SMC-240 was sufficient for detection of ENaC beta in 15 µg of Mouse whole kidney lysate by ECL immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Tissue: Kidney (cortex). Species: Mouse. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:150. Localization: Collecting duct principal cells. Magnification: 60X.

Western Blot analysis of Mouse Whole kidney homogenates showing detection of ~87kDa ENaC beta protein using Mouse Anti-ENaC beta Monoclonal Antibody, Clone 7B8. Lane 1: Molecular Weight Ladder (MW). Lane 2: Low-salt diet. Lane 3: Normal-salt diet. Load: 20 μg. Primary Antibody: Mouse Anti-ENaC beta Monoclonal Antibody at 1:1000. Predicted/Observed Size: ~87kDa.