ENaC gamma Antibody: Biotin

• Catalog Number: orb152751
• Category: Antibodies
• Description: Rabbit polyclonal to ENaC Gamma (Biotin). The Epithelial Sodium Channel (ENaC) is a membrane ion channel permeable to Na+ ions. It is located in the apical plasma membrane of epithelia in the kidneys, lung, colon, and other tissues where it plays a role in trans epithelial Na+-ion transport. Specifically Na+ transport via ENaC occurs across many epithelial surfaces, and plays a key role in regulating salt and water absorption. ENaCs are composed of three structurally related subunits that form a tetrameric channel, alpha, beta , and gamma. The expression of its alpha and beta subunits is enhanced as keratinocytes differentiate. The beta and gamma-ENaC subunits are essential for edema fluid to exert its maximal effect on net fluid absorption by distal lung epithelia(5). And it has been concluded that the subunits are differentially expressed in the retina of mice with ocular hypertension, therefore the up-regulation of alpha-ENaC proteins could serve as a protection mechanism against elevated intraocular pressure..
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC, WB
• Reactivity: Frog, Hamster, Human, Mouse, Rat
• Immunogen: Produced against the C-terminal tail (amino acids 629-650) of rat gamma ENaC (antibody designation L550)
• Concentration: 1 mg/ml
• Dilution range: WB (1:1000), IHC (1:100)
• Conjugation: Biotin
• MW: 83kDa
• Target: ENaC
• Entrez: 24768
• UniProt ID: P37091
• NCBI: NP_058742
• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 136.36mM Ethanolamine, and 9.55mM Sodium Bicarbonate in 95.45% PBS
• Alternative names: SCNN1G antibody, Epithelial Sodium Channel gamma a
• Note: For research use only
• Application notes: 1 µg/ml of SPC-405 was sufficient for detection of gamma-ENaC in 20 µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry analysis using Rabbit Anti-ENaC Polyclonal Antibody. Tissue: kidney tissue. Species: Rat. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:100. Secondary Antibody: FITC Goat Anti-Rabbit (green).

Western blot analysis of Rat kidney tissue lysates showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000.

Western blot analysis of Mouse kidney cortex showing detection of ENaC protein using Rabbit Anti-ENaC Polyclonal Antibody. Primary Antibody: Rabbit Anti-ENaC Polyclonal Antibody at 1:1000. Low-salt diet (lanes 1-4) compared to a high-salt diet (lanes 5-7). 70kDa degradation band observed in low-salt.