EN2 Antibody

SKU: orb1263208

Description

EN2 Antibody

Research Area

Neuroscience, Signal Transduction

Images & Validation

• Tested Applications: FC, IHC-P, WB
• Reactivity: Human
• Application Notes:
  For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Immunogen: This EN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human EN2.
• Target: EN2
• Molecular Weight: 34 kDa
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Homeobox protein engrailed-2, Homeobox protein en-2, Hu-En-2, EN2

Western blot analysis of EN2 antibody (C-term-2) in NCI-H460 cell line lysates (35 ug/lane)

Formalin-fixed and paraffin-embedded human brain with EN2 Antibody (C-term-2), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Quick Database Links

Gene Symbol

EN2

UniProt

P19622

RefSeq:P19622