Goat anti-Engrailed-2 Antibody

• Catalog Number: orb19185
• Category: Antibodies
• Description: Goat polyclonal antibody to EN2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Conjugation: Unconjugated
• MW: 34.2
• Target: Engrailed-2
• Entrez: 2020
• Protein Sequence: NHSTTAKEGKSDSE
• RRID: AB_10753306
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti Engrailed-2 antibody, anti engrailed homolog
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 30kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2kDa according to Human NP_001418.2 and of 33.8kDa according to Mouse NP_034264.1 and of 33.7kDa according to Rat NP_001102684.1). Recommended concentration: 0.1-0.3 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb19185 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Mouse Brain lysate using EN2 antibody