Engrailed-2 Antibody

SKU: orb19185

Description

Goat polyclonal antibody to EN2

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, WB
• Dilution Range: ELISA: 1:4000, WB: 0.1-1 μg/ml, IHC-P: 10 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx. 30kDa band observed in Mouse and Rat Brain lysates (calculated MW of 34.2kDa according to Human NP_001418.2 and of 33.8kDa according to Mouse NP_034264.1 and of 33.7kDa according to Rat NP_001102684.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Host: Goat
• Clonality: Polyclonal
• Target: Engrailed-2
• Protein Sequence: NHSTTAKEGKSDSE
• Molecular Weight: 34.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti Engrailed-2 antibody, anti engrailed homolog 2 antibody, anti EN2 antibody, anti AUTS1 antibody

orb19185 (0.1ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Mouse Brain lysate using EN2 antibody

Quick Database Links

Gene Symbol

Engrailed-2

NCBI Gene ID

2020