EN2 Rabbit Polyclonal Antibody

SKU: orb329598

Description

Rabbit polyclonal antibody to EN2

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Equine, Guinea pig, Mouse, Rat, Zebrafish

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The immunogen is a synthetic peptide directed towards the C-terminal region of Human EN2
• Target: EN2
• Protein Sequence: Synthetic peptide located within the following region: NESQIKIWFQNKRAKIKKATGNKNTLAVHLMAQGLYNHSTTAKEGKSDSE
• Molecular Weight: 34kDa
• Purification: Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Sample Tissue: Human Jurkat, Antibody Dilution: 1.0 ug/mL.

Positive control (+): 293T (2T), Negative control (-): Ovary tumor (T-OV), Antibody concentration: 3 ug/mL.

Human Kidney

Rabbit Anti-EN2 Antibody, Paraffin Embedded Tissue: Human Kidney, Cellular Data: Epithelial cells of renal tubule, Antibody Concentration: 4.0-8.0 ug/mL, Magnification: 400X.

WB Suggested Anti-EN2 Antibody Titration: 0.05 ug/mL, ELISA Titer: 1:2500, Positive Control: Human cerebellum.

Quick Database Links

Gene Symbol

EN2

UniProt

P19622

RefSeq (Protein):NP_001418