You have no items in your shopping cart.
Featured
Description
Research Area
Immunology & Inflammation, Signal Transduction
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, IP, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1). |
| Target | EIF4E |
| Molecular Weight | Observed: 25kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Eukaryotic translation initiation factor 4E, eIF-4E, eIF4E, eIF-4F 25 kDa subunit, mRNA cap-binding protein, EIF4E, EIF4EL1, EIF4F
Similar Products
−- Item 1 of 5
- Item 1 of 5
Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody [orb13390]
FC, IF, IHC-Fr, IHC-P, WB
Bovine, Canine, Equine, Gallus, Porcine, Rabbit, Zebrafish
Human, Mouse, Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 4
4E-BP1 (phospho Thr69) rabbit pAb Antibody [orb767260]
ELISA, IF, IHC, WB
Mouse, Rat
Polyclonal
Unconjugated
50 μl, 100 μl - Item 1 of 1
Rat Eukaryotic Initiation Factor 4E (eIF4E) ELISA Kit [orb782346]
Rat
93.75-6000 pg/mL
26.14 pg/mL
48 T, 96 T - Item 1 of 5
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Western blot analysis of extracts of various cell lines, using EIF4E antibody (orb1258245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using EIF4E antibody (orb1258245) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug EIF4E antibody (orb1258245). Western blot was performed from the immunoprecipitate using EIF4E antibody (orb1258245) at a dilution of 1:1000.
Quick Database Links
Gene Symbol
EIF4E
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
Request a Document
Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
IP
Immunoprecipitation
EIF4E Antibody (orb1258245)
- 0.0
Based on 0 reviews
Participating in our Biorbyt product reviews program enables you to support fellow scientists by sharing your firsthand experience with our products.
Login to Submit a ReviewAvailable Sizes
Select a size below
Choose Conjugation or Carrier Free Version
Free Secondary Antibody (20 ul)0/0
Please add an antibody product to your cart first.
Usually dispatched within 5-10 working days