EIF4E Antibody

• Catalog Number: orb1258245
• Category: Antibodies
• Description: EIF4E Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human EIF4E (NP_001959.1).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 25kDa
• Target: EIF4E
• UniProt ID: P06730
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: Eukaryotic translation initiation factor 4E, eIF-4
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using EIF4E antibody (orb1258245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human lung cancer using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human stomach using EIF4E antibody (orb1258245) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using EIF4E antibody (orb1258245) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug EIF4E antibody (orb1258245). Western blot was performed from the immunoprecipitate using EIF4E antibody (orb1258245) at a dilution of 1:1000.