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Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Catalog Number: orb13390

DispatchUsually dispatched within 5-10 working days
$ 210.00
Catalog Numberorb13390
CategoryAntibodies
DescriptionPhospho-eIF4E (Ser209) Rabbit Polyclonal Antibody
TargetEIF4E
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationUnconjugated
ReactivityHuman, Mouse, Rat, Zebrafish
Predicted ReactivityBovine, Canine, Equine, Gallus, Porcine, Rabbit
Form/AppearanceLiquid
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated Synthesised phosphopeptide derived from human eIF4E around the phosphorylation site of Ser209 SG(p-S)TT
UniProt IDP06730
RRIDAB_10751395
MW24 kDa
Tested applicationsFC, IF, IHC-Fr, IHC-P, WB
Dilution rangeWB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=0.2ug/test
Antibody TypePrimary Antibody
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Alternative nameseIF4E(phospho S209); eIF4E(phospho Ser209); p-eIF4
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NoteFor research use only
Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Blank control: Molt-4. Primary Antibody (green line): Rabbit Anti-phospho-eIF4E (Ser209) antibody (orb13390), Dilution: 0.2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-PE, Dilution: 0.2 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 20% PBST for 20 min at -20°C. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-eIF4E (Ser209)) Polyclonal Antibody, Unconjugated (orb13390) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-eIF4E (Ser209)) Polyclonal Antibody, Unconjugated (orb13390) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (rat testis), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (phospho-eIF4E (Ser209)) Polyclonal Antibody, Unconjugated (orb13390) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Sample: NIH/3T3 (Mouse) Cell Lysate at 30 ug, Primary: Anti-phospho-eIF4E (Ser209) (orb13390) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 24 kD, Observed band size: 28 kD.