EIF4E2 Antibody

SKU: orb1932244

Description

Affinity Purified Rabbit Polyclonal Antibody (Pab)

Research Area

Infectious Disease & Virology, Molecular Biology, Protein Biochemistry, Signal Transduction

Images & Validation

• Tested Applications: IHC-P, WB
• Dilution Range: WB - 1:2000, IHC-P - 1:25
• Reactivity: Human, Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.
• Target: EIF4E2 {ECO:0000303|PubMed:15153109, ECO:0000312|HGNC:HGNC:3293}
• Molecular Weight: 28362 Da
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Eukaryotic translation initiation factor 4E type 2, eIF-4E type 2, eIF4E type 2, Eukaryotic translation initiation factor 4E homologous protein, Eukaryotic translation initiation factor 4E-like 3, eIF4E-like protein 4E-LP, mRNA cap-binding protein 4EHP, mRNA cap-binding protein type 3, EIF4E2, EIF4EL3

Staining EIF4E2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining EIF4E2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: RD whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: RD whole cell lysate. Lane 5: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28, 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: RD whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28, 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Quick Database Links

Gene Symbol

EIF4E2 {ECO:0000303|PubMed:15153109, ECO:0000312|HGNC:HGNC:3293}

UniProt

O60573

RefSeq (Protein):NP_004837.1