EIF4E2 Antibody

• Catalog Number: orb1932244
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB22944
• Tested applications: IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:2000, WB: 1:2000, WB: 1:2000, IHC-P: 1:25, IHC-P: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 28362 Da
• Target: This EIF4E2 antibody is generated from rabbits immunized with human EIF4E2 recombinant protein.
• UniProt ID: O60573
• NCBI: NP_004837.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Eukaryotic translation initiation factor 4E type 2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Staining EIF4E2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Staining EIF4E2 in human stomach tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: RD whole cell lysate. Lane 3: Hela whole cell lysate. Lane 4: HepG2 whole cell lysate. Lane 5: HL-60 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: Hela whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: RD whole cell lysate. Lane 5: C2C12 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28, 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

All lanes: Anti-EIF4E2 Antibody at 1:2000 dilution. Lane 1: 293 whole cell lysate. Lane 2: HepG2 whole cell lysate. Lane 3: HL-60 whole cell lysate. Lane 4: RD whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 28, 27 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.