EIF3S2 Polyclonal Antibody

SKU: orb1420014

Description

Rabbit polyclonal antibody to EIF3S2.

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, ICC, IF, IHC-Fr, IHC-P, WB
• Reactivity: Porcine, Rat

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Disclaimer: For research use only

Alternative Names

Eukaryotic translation initiation factor 3 subunit I {ECO:0000255|HAMAP-Rule:MF_03008}, eIF3i {ECO:0000255|HAMAP-Rule:MF_03008}, Eukaryotic translation initiation factor 3 subunit 2 {ECO:0000255|HAMAP-Rule:MF_03008}, TGF-beta receptor-interacting protein 1, TRIP-1, eIF-3-beta {ECO:0000255|HAMAP-Rule:MF_03008}, eIF3 p36 {ECO:0000255|HAMAP-Rule:MF_03008}, EIF3I {ECO:0000255|HAMAP-Rule:MF_03008}

Sample: U937 (Human) Cell Lysate at 30 ug. Primary: Anti- EIF3S2 at 1/1000 dilution. Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution. Predicted band size: 37 kD. Observed band size: 35 kD.

Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Incubation: Anti-EIF3S2 Polyclonal Antibody, Unconjugated 1: 200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

UniProt

Q13347