EHD2 Antibody

• Catalog Number: orb1248621
• Category: Antibodies
• Description: EHD2 Antibody
• Target: EHD2
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Immunogen: The immunogen for this antibody is: CRLVPPSKRRHKGSA
• UniProt ID: Q9NZN4
• Tested applications: ELISA, FC, IF, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 65kDa band observed in Human Placenta lysates (calculated MW of 61.2kDa according to NP_055416.2). Recommended concentration: 0.1-0.3ug/ml. Primary incubation was 1 hour.Immunofluorescence: strong expression of the protein seen in the plasma membrane of A431 cells and the nuclei of HeLa cells. Recommended concentration: 10µg/ml.Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: EHD2, PAST2, EH-domain containing 2, EH domain con
• Note: For research use only
• NCBI: NP_055416.2

orb1248621 (0.1 μg/ml) staining of A549 (A) and (0.3 μg/ml) HeLa (B) cell lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.

orb1248621 (0.3 μg/ml) staining of Human Lung lysate (35 μg protein in RIPA buffer). Detected by chemiluminescence.