EHD2 Antibody (C-term)

• Catalog Number: orb1926709
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Target: This EHD2 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 415-449 amino acids from the C-terminal region of human EHD2.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• UniProt ID: Q9NZN4
• MW: 61161 Da
• Tested applications: IHC-P, WB
• Dilution range: WB: 1:1000, WB: 1:500-1:2000, IHC-P-Leica: 1:500, IHC-P-Leica: 1:500
• Antibody Type: Primary Antibody
• Clone Number: RB51848
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: EH domain-containing protein 2, PAST homolog 2, EH
• Note: For research use only

All lanes: Anti-EHD2 Antibody (C-term) at 1:500-1:2000 dilution. Lane 1: A549 whole cell lysate. Lane 2: Human heart tissue lysate. Lane 3: Mouse lung tissue lysate. Lane 4: Rat lung tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded human heart tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human placenta tissue was performed on the Leica BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9.0). Samples were incubated with primary antibody (1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

All lanes: Anti-EHD2 Antibody (C-term) at 1:1000 dilution. Lane 1: Human heart tissue lysate. Lane 2: A549 whole cell lysate. Lane 3: Human placenta tissuee lysate. Lane 4: Hela whole cell lysate. Lane 5: Mouse kidney tissue lysate. Lane 6: Rat lung tissue lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.