Goat anti-EHD2 Antibody

• Catalog Number: orb18895
• Category: Antibodies
• Description: Goat polyclonal antibody to EHD2
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 10ug/ml
• Conjugation: Unconjugated
• MW: 61.2
• Target: EHD2
• Entrez: 30846
• Protein Sequence: CRLVPPSKRRHKGSA
• RRID: AB_10749750
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti EHD2 antibody, anti PAST2 antibody, anti EH-d
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in Human Heart lysates (calculated MW of 61.2kDa according to NP_055416.2). In transfected HEK293 transiently expressing EHD2 a band of approx. 62kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.003-0.01 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18895 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb18895 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18895 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

orb18895 (0.1g/ml) staining of Human Lung (A), Colon (B) and Placenta (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.